Exploring the Potential of Deep Eutectic Solvents in Concentrating Non-enveloped Virus for Improving Rapid Virus Detection

Presenter

Lily Elias Saad

Campus

UMass Amherst

Sponsor

Matthew D. Moore, Department of Food Science, UMass Amherst

Schedule

Session 4, 2:30 PM - 3:15 PM [Schedule by Time][Poster Grid for Time/Location]

Location

Poster Board A12, Campus Center Auditorium, Row 1 (A1-A20) [Poster Location Map]

Abstract

Introduction: Concentration of noroviruses from foods is a crucial step for effective viral detection. Deep Eutectic Solvents (DESs) are an emerging class of reagents that are composed of Lewis or Bronsted acids and bases that form eutectic mixtures that have adjustable properties, including charge, depending on the ratios of the components in the solvent. Both synthesis and purification of DESs is environmentally friendly and cost-efficient. Although promising, the potential of DESs to serve as a reagent for concentration of noroviruses prior to detection has not been evaluated.

Purpose: The purpose of this study was to examine the ability of DESs to concentrate bacteriophage MS2, a human norovirus surrogate.

Methods: MS2 was diluted to 10^5 PFU/mL in PBS, vortexed with different DESs (Cobalt, Nickel, and Dysprosium-based), and separated using a magnet. The supernatant was removed and samples were washed to remove the unbound virus. Captured MS2 was eluted by vortexing with modified Luria Broth, and recovered virus quantified by RT-qPCR.

Results: The Cobalt-based DES displayed 65.8% capture and 29.8% recovery; the Nickel-based DES displayed 60% capture and 36% recovery; and the Dysprosium-based DES displayed 99% capture but only 0.53% recovery (n=3). Thus, the DESs displayed promising capture results, though recovery was notably lower for all DESs, especially dysprosium, suggesting that further optimization of elution conditions could improve recovery.

Significance: These results indicate that DESs have the potential to recover significant amounts of non-enveloped viruses and have potential to serve as a green, cost-effective reagent for concentration of viruses from food and environmental samples.

Keywords

Deep Eutectic Solvents (DESs), Virus Capture, Virology, Sustainability , Rapid Detection

Research Area

Agriculture and Agronomy / Food Science

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