Antibiotic resistance is a growing issue for public health. The need for novel antibiotics able to fight resistant infections is increasing constantly. The solution for this public health crisis may lie just beneath our feet; soil bacteria are incredibly common antibiotic producers, as soil is so densely packed with bacteria, they use antibiotics to fight for resources and space in the soil. However, this is not as cut-and-dry a solution as it seems; millions of bacteria make their home in soil, and most antibiotics produced are ineffective against modern pathogens or unsafe for human treatments. This project aims to discover a novel antibiotic from a soil bacterium that is effective against one or more ESKAPE bacteria (Enterococcus species, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, Enterobacter species). ESKAPE bacteria are some of the most common species responsible for nosocomial infections, and all are increasingly resistant to modern antibiotic treatments. Through this research, we discovered SR-01-25, an antimicrobial soil bacterium that is effective against S. aureus, K. pneumoniae, and P. aeruginosa. We hope to identify the species of SR-01-25 through 16S rRNA sequencing, and to chemically analyse the chemical it produces to identify it as an existing or novel antibiotic.