Signaling pathways are crucial to keeping any organism’s internal balance in check, being responsible for everything from response to environment to maintaining balance within any living organism. Oxidative stress is a situation that disrupts an organism’s balance, resulting in an increase in the amount of unstable molecules known as free radicals. The damage free radicals cause is vast, typically shortening an organism's lifespan or contributing to chronic illnesses. A gene known as Enigma encodes a protein located within the mitochondria classified as oxidoreductase, which prevents oxidative stress from occurring and is responsible for regulating the homeostasis of lipids. Mutations in the Enigma gene can cause lost or reduced functions of the production of oxidoreductase. The objective of this project is to research the presence of Enigma in other Drosophila species that are not as well annotated as D. melanogaster. The two species of interest in this research project are D. bipectinata and D. willistoni. After the necessary data is collected, a model will be constructed to show all three of the annotated genes of the species, showing how Enigma manifests across different species under the same genus. The Genomics Education Partnership online database will be used to annotate  genomes of interest in the research of this project. This research on the synteny of D. melanogaster with D. bipectinata and D. willistoni is a contribution to the 2026 Genomics Education Partnership Pathways project, exploring how genes in pathways evolve.