Antibiotic resistance is an increasing global health concern. As part of the Tiny Earth initiative, soil samples were collected and cultured to isolate environmental bacteria with potential antibiotic activity. Bacterial isolates were screened for antimicrobial activity against Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis. Two isolates (8 and 10) showed inhibitory activity: isolate 8 inhibited Staphylococcus epidermidis, whereas isolate 10 inhibited Escherichia coli, Bacillus subtilis, and Staphylococcus epidermidis. Both isolates were subsequently selected for further characterization.  Isolate 8 was a non-motile, Gram-positive rod, cream to white in color, that fermented glucose and xylose without gas production and tested negative for oxidase and positive for catalase suggesting Bacillus or possible Paenibacillus species. Isolate 10 presented as a non-motile, Gram-negative rod exhibiting orange pigmentation. The isolate fermented glucose and sucrose without gas production and yielded negative oxidase-negative and positive catalase reactions suggesting Pantoea species. Bacterial identification is currently being completed using 16s rRNA sequencing. Overall, this study identified two soil isolates with inhibitory activity against indicator organisms, showing the use of soil screening as an effective approach for discovering potential antibiotic producing bacteria.This study shows how course-based research experiences supports the efforts against antibiotic resistance by allowing students to identify soil bacteria that produce compounds capable of inhibiting bacterial growth.