Presenter: Aaron Ross Cashton
Faculty Sponsor: Ana Caicedo
School: UMass Amherst
Research Area: Biology
Session: Poster Session 6, 4:15 PM - 5:00 PM, 165, D1
ABSTRACT
The genus, Cuscuta, consists of over 200 species of parasitic plants that lack leaves and roots and are distributed across the world. As a holoparasitic plant, Cuscuta species cannot carry out photosynthesis and wrap themselves around the host plant stem while absorbing nutrients via haustorial penetration. The species, Cuscuta campestris, also known as field dodder, is prevalent in New England, often found in swampland and disturbed areas. Most plant species associate with microbes that can provide services to the plants. Much of this interaction takes place in the rhizosphere, the root area, or the phyllosphere, the leaf area, both of which dodder lacks. Microbes, termed endophytes, can also exist within plant tissues. Recent studies have examined Cuscuta’s endophytic interactions to determine the transfer of microbes that may relate to the host plant's stress response and nutrient uptake during parasitization. To date, dodder’s native endophytic environment prior to parasitization or influence from soil has not been examined. In this study, we aim to characterize the endophytic community of Cuscuta campestris without the influence of hosts or soil. We germinated and surface sterilized 8 replicates of 40 C. campestris seedlings grown in sterile petri dishes for one week. Upon DNA extraction, we amplified and sequenced 16S ribosomal RNA genes to characterize the microbial data in C. campestris strands. Our results will provide a clearer understanding of what microbes are present in dodder seedlings prior to attachments to hosts and what role they may play in the growth and development of the parasite.