Regulatable RNA Probes: Toward Spatiotemporal Control of Cellular RNA Granule Formation

Presenter

Justin Kieu

Campus

UMass Amherst

Sponsor

Mingxu You, Department of Chemistry, UMass Amherst

Schedule

Session 4, 2:30 PM - 3:15 PM [Schedule by Time][Poster Grid for Time/Location]

Location

Poster Board A67, Campus Center Auditorium, Row 4 (A61-A80) [Poster Location Map]

Abstract

RNA localization plays a pivotal role in various cellular processes. Transcription, splicing, RNA degradation, heterochromatin formation, and stress response are all influenced by RNA localization. Therefore, manipulating RNA localization through macromolecular condensates may offer a promising avenue for regulating gene expression. This research aims to establish a method for controlling RNA granule formation using a tetracycline-based small molecule regulator via antisense oligonucleotide, coupled with a small molecule binding aptamer. Leveraging the inherent condensate forming capability of CAG trinucleotide repeats, we will investigate the inhibitory potential of CUG repeats at varying concentrations and lengths in order to seek the critical length for effective inhibition. Subsequently, an aptamer wil be designed based on the critical length and tested in vitro. If successful, a tetracycline-regulated aptamer will be developed and evaluated in both vitro and in vivo to visualize and regulate RNA granule condensation. The ultimate goal is to achieve spatiotemporal control over RNA granule formation and gain insights into granule dynamics within different signaling pathways.

Keywords

Gene Expression, Granules, Small Molecules, RNA Probes, Tetracyline

Research Area

Chemistry and Materials Science

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