Mechanical Characterization of Murine Kidney, Liver, and Spleen Using Needle-Induced Cavitation

Presenter

Hsu Shwe Yee Naing

Campus

UMass Amherst

Sponsor

Shelly Peyton, Department of Chemical Engineering, UMass Amherst

Schedule

Session 2, 11:30 AM - 12:15 PM [Schedule by Time][Poster Grid for Time/Location]

Location

Poster Board A85, Campus Center Auditorium, Row 5 (A81-A100) [Poster Location Map]

Abstract

The mechanical properties of biological tissues serve an important purpose as biomarkers for various physiological conditions, including cancer diagnosis and disease progression. Compared to traditional bulk mechanical characterization techniques such as tensile and compression tests, needle-induced cavitation (NIC) can be used to detect local heterogeneities in mechanical properties within the tissue at low strain rates. The technique involves creating a cavity at the tip of a syringe needle in different regions of the tissues and relating the pressure recorded to the moduli values. 

In this study, murine kidney, liver, and spleen tissue were tested at different post-mortem times to observe the effect of post-mortem time on the modulus value. The results indicate that tissues subjected to longer post-mortem time exhibited statistically lower modulus values compared to those tested immediately after harvesting, reflecting tissue degradation. Specifically, the moduli of murine kidney, liver, and spleen immediately after harvesting were measured at 1.31±0.13 kPa, 1.29±0.24 kPa, and 1.39±0.30 kPa, respectively. However, after 4 hours of refrigeration, there was a substantial decrease in modulus values, with measurements of 0.68±0.15 kPa for kidneys, 0.89±0.37 kPa for murine liver, and 0.68±0.08 kPa for murine spleen, indicating significant tissue softening due to degradation. These findings demonstrate the importance of conducting mechanical testing of tissues promptly after harvesting or after cryopreservation protocols. 

Keywords

Mechanical Characterization, Soft Materials, Needle-Induced Cavitation

Research Area

Engineering

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