A Direct Co-culture Model to Examine the Influence of Prostate Cancer Cell Lines on Bone Resorption

Presenter

Jack Muse

Campus

UMass Amherst

Sponsor

Stacyann Bailey, Department of Biomedical Engineering, UMass Amherst

Schedule

Session 5, 3:30 PM - 4:15 PM [Schedule by Time][Poster Grid for Time/Location]

Location

Poster Board C38, Poster Showcase Room (163), Row 4 (C31-C40) [Poster Location Map]

Abstract

Prostate cancer, the most common primary cancer among men, frequently metastasizes to bone and causes debilitating symptoms including bone pain and increased risk of pathologic fractures. However, different prostate cancer cell lines result in different metastatic lesions, and the influence of specific prostate cancer cell lines on bone-resorbing osteoclast activity in the bone microenvironment is not completely understood. This gap in understanding hinders the development of targeted interventions to mitigate bone fragility. This project aims to examine two human prostate cancer cell lines with phenotypically distinct lesion types, PC-3 and MDA-PCa-2b, and their influence on bone resorption.

PC-3 and MDA-PCa-2b prostate cancer cell lines were cultured in various media types at different seeding densities and monitored over a 7-day period. PC-3 cells were viable in osteoclast differentiation media, but MDA-PCa-2b required a 1:1 mixture of bone-resorbing osteoclast differentiation media and prostate cancer cell media to maintain viability over the 7-day period. Additionally, human peripheral blood mononuclear cells (PBMCs) were isolated from whole blood and differentiated into osteoclasts. Distinct changes in osteoclast morphology and sustained viability were observed over the 14-day differentiation period, with morphological changes confirmed by tartrate-resistant acid phosphatase staining to visualize osteoclast multinuclearity. Future work involves co-culturing PBMC-derived osteoclasts with PC-3 or MDA-PCa-2b on bone slices to assess their impact on bone resorption. Protein biomarkers and gene expression of osteoclastic and metastatic activity will be quantified over time to elucidate the osteoclast-prostate cancer cell relationship in bone metastasis.

Keywords

prostate cancer, bone metastasis, osteoclast, biomedical engineering, cell culture

Research Area

Cancer Studies

SIMILAR ABSTRACTS (BY KEYWORD)